WebDAPI is a fluorescent dye that binds selectively to double-stranded DNA and forms strongly fluorescent DNA-DAPI complexes with high specificity. [ 1 [ 2] [ 3] [ 4] It is commonly used to detect mycoplasma in cell culture via fluorescence microscopy. [ 5] DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. WebThe 5 mg/mL DAPI stock solution may be stored at 2–6°C for up to 6 months or at ≤–20°C for longer periods. 2. Add 2.1 µL of the 14.3 mM DAPI stock solution to 100 µL PBS to make a 300 µM DAPI intermediate dilution. 3. Add 10 µL of the 300 µM DAPI intermediate dilution to 10 mL PBS to make a 300 nM DAPI stain solution.
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WebEspecialista em inovação, design estratégico e modelagem de negócios criativos. Combino processos de validação, agilidade e experiência do usuário para impulsionar a Transformação Digital e Cultural em grandes corporações, startups e projetos que impactam a sociedade. Experiências em projetos de inovação como consultor e … WebDAPI is a popular nuclear counterstain for use in multicolor fluorescent techniques. Its blue fluorescence stands out in vivid contrast to green, yellow, or red fluorescent probes of … chavo shirt
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WebWorld class Antigens from BBI BBI Solutions is the world’s primary manufacturer of high quality native human antigens, to a variety of disease states. Our native antigens are purified from human plasma and tissues which are all processed at our HTA registered site in … WebImmune mechanisms that regulate the blood-brain-interface (BBI) Immune factors that affect health psychology Sleep, exercise, immunity and health Immune system interactions that affect behavior following use of psychotropic drugs, alcohol and other drugs of abuse Healthy aging of the immune system and brain WebTreat your cells with or without Verapamil and then do immunofuluorescence for your protein with DAPI staining. The Verapamil treated cells should have DAPI in "all cells" but verapamil untreated ... chavos meaning