WebHorse serum has less protein content and fatty acid content than FBS. I tried to culture MCF10A once with FBS and another with horse serum. Cells were growing much faster in DMEM/F12 media ... WebDec 1, 2024 · Prior to transfection, HEK293T cells were cultured at 37 °C in DMEM (SparkJade, China) supplemented with 10% fetal bovine serum (FBS) (BioInd, Israel). 24 h before transfection, cells were seeded into 24-well plates at the concentration of 1 × 10 5 per well. Then, 500 ng of TNFα promoter construct of different lengths in pGL3-basic, 300 ng ...
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WebAug 18, 2024 · The GES-1 cells were treated with FBs (0–40 µM) for 48 h, then they were digested 0.25% trypsin (BioInd) and harvested by centrifugation (1000 rpm/min for 5 min). The cells were stained with 5 μL PI and 5 μL Annexin V … WebApr 10, 2024 · MKN28, MKN45, HGC27 and GES-1 cells were cultured in RPMI-1640 medium (BioInd, Israel) and AGS cells were cultured in DMEM (BioInd, Israel) with 10% fetal bovine serum (FBS) (BioInd, Israel) and 1 ... chi touchscreen blow dryer
Autoserum: An Optimal Supplement for Bone Marrow Mesenchymal ... - Hindawi
WebThe suspension was centrifugated, and the supernatant was collected in cold basal medium containing fetal bovine serum (FBS) (Bioind) and antibiotics (Penicillin-Streptomycin, 100 U/ml) (HyClone). After centrifugation, the resulting pellet was resuspended in the complete medium and filtered through a 40-μm nylon cell strainer. WebDCCM-2: A serum-free medium, designed for hybridoma cell growth and monoclonal antibody production. The medium is a low protein formulation, and it is ready-to-use and needs only the addition of L-Glutamine. DCCM-2 contains BSA. LPM (Low Protein Media): This media is a Bovine Serum Albumin (BSA) free formulation. WebBrothers-in-Farms (BiF) is an elite cannabis-focused community of testers, breeders, product and content producers, and other like-minded individuals who share a common passion for procuring and spreading the best cannabis genetics the world has to offer. We have breeders, vendors , and testers from all around the world that are working ... grass catcher john deere s240