WebIs there any open-source software to do analysis of bands in the gel resulting from DGGE? My goal in my research is to analyse the dynamics of bacterial community structures in … WebSvein Valla. TrfA is the only plasmid-encoded protein required for RK2 replication. We report here the construction and characterization of an RK2-based vector in which trfA is …
PCR-DGGE analysis for the identification of microbial populations from ...
WebMar 1, 2016 · The similarities of the PCR-DGGE DNA profiles were analyzed using Phyltools Analysis Software (Joseph Felsenstein, University of Washington, version 1.32). Dendrograms were constructed by the unweighted pair-group method with arithmetic mean and unweighted pair-group method with arithmetic cluster analysis of the microorganism … WebThe software can measure average and total quantities and determine relative and actual amounts of proteins. Gel imaging software is also capable of determining the presence … noreen godwin snow hill md
Temperature gradient gel electrophoresis - Wikipedia
WebWe can use DGGE to identify the species present from field samples to narrow down the possible causes and then sequence the DNA fragments to definitively identify the culprit. To start, we isolate DNA from field … WebJul 30, 2024 · The haloarchaeal diversity of four hypersaline alkaline lakes from the Wadi El-Natrun depression (Northern Egypt) was investigated using culture-independent polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rRNA gene phylotypes, which was combined with remote sensing and geographic information … WebNov 19, 2009 · The microbial diversity of Japanese- and Chinese-fermented soybean pastes was investigated using nested PCR–denaturing gradient gel electrophoresis (DGGE). Five Japanese-fermented soybean paste samples and three Chinese-fermented soybean paste samples were analyzed for bacteria and fungi. Extracted DNA was used … how to remove hairspray buildup in bathroom