WebOpti-MEM reduced serum medium, no phenol red (Life Technologies, Cat. No. 11058-021) 3. Sucrose (Sigma-Aldrich, Cat. No. S0389) 4. 0.1% fibronectin solution from human plasma (Sigma-Aldrich, Cat. No. F0895) 5. Gelatin powder from bovine skin (Sigma-Aldrich, Cat. No. G9391) 6. RNase-free water (Fisher BioReagents, Cat. No. 7732-18-5) 7. WebJan 20, 2024 · Lipofectamine (Invitrogen) and 200 nM siRNAs were mixed in Opti-MEM (Thermo Scientific, Rockford, IL, USA). After 48 h of EZH2-siRNA transfection, the RNA …
Process for an efficient lentiviral cell transduction Biology …
WebOpti-MEM® I Reduced-Serum Medium is a chemically defined, low protein, Minimal Essential Medium (MEM) containing insulin, transferrin, hypoxanthine, thymidine, and trace … WebJun 27, 2024 · So, you need to form your lipofectamine-DNA interaction in serum-free media or OptiMEM (preferred), leave for 15 min as mentioned in the protocol, and add to the serum containing media in the ... hovercraft plans diy
Lipofectamine - an overview ScienceDirect Topics
WebAfter 4 h of post-transfection, the cells were washed once with phosphate-buffered saline (PBS) solution (Thermo Fisher Scientific, Waltham, MA, USA), followed by media changed to OptiMEM Reduced serum medium (Thermo Fisher Scientific, Waltham, MA, USA). After 48 h postconditioning, the EVs were isolated as described below. WebOpti-MEM® Reduced Serum Medium ∤ Eppendorf tubes Timing Preparation: 10 minutes Incubation: 5 minutes Final Incubation: 1-3 days Selection Guide Lipofectamine® Reagents Go online to view related products. Product Description ∤ Lipofectamine® RNAiMAX Transfection Reagent is a proprietary formulation for transfecting small RNAs (e.g., WebJun 25, 2024 · Human embryonic kidney (HEK) 293 cells and HeLa cells (both DSMZ, Braunschweig, Germany) were grown in Dulbecco’s modified Eagles medium (DMEM; Thermo Fisher Scientific) supplemented with 10% FCS, streptomycin (100 U/mL), penicillin (100 μg/mL) at 37 °C in a water saturated atmosphere containing 5% CO 2. hovercraft plans free