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WebProperties. Concentration: 5x Hot-start: no Ready to load: yes Fidelity: 1x Taq Cloning Type: T/A Cloning Amplicon Sixe: up to 5 kb Mix Components. FIREPol ® DNA polymerase: highly processive. thermostable DNA polymerase. 5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH 4) 2 SO 4, 0.1% w/v Tween-20 7.5 mM MgCl 2: 1x PCR solution – 1.5 mM MgCl 2. 1 mM … WebNow a junior at Wilmington University, Wilmington, Del., she’s back on the court “and is 100 percent ready to go.”. She looks forward to an active basketball season. Now 20, Tanasia … WebDescription. HOT FIREPol ® MultiPlex Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for hot-start multiplex PCR (except template, primers, and water) and an additional compound needed for direct loading onto an agarose gel and two tracking dyes (blue and yellow) that allow monitoring progress during … imf article iv singapore