Thy1-gcamp6 transgenic mice
Webb16 feb. 2024 · Thy1-GCaMP6s transgenic mice have been developed by transgenic meth- ods and express GCaMP6s under the Thy1promoter, in which projection neurons in the cerebral cortex and hippocampus... Webb11 okt. 2024 · Transgenic mice expressing the green GECI GCaMP6 are already widely used. Here we present the generation and characterization of transgenic mice …
Thy1-gcamp6 transgenic mice
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WebbThy1 -GCaMP6-WPRE transgenic mice were generated using standard techniques [23].We included the WPRE (Woodchuck hepatitis virus post-transcriptional regulatory element), … Webb7 jan. 2024 · In this study, we describe an experimental procedure for measuring calcium dynamics through dual optical windows above bilateral primary somatosensory corticies …
Webb1 jan. 2012 · It is shown that GCaMP6 Thy1 transgenic mice are useful for long-term, high-sensitivity imaging in behaving mice and are shown to be effective for in vivo imaging of genetically defined neuronal populations in vivo. 407 PDF View 2 excerpts, cites background DEVELOPMENT OF A NONINVASIVE CALCIUM IMAGING PROBE WebbNew generations of Thy1-XFP mice are now being generated at a high rate, and these have allowed previously difficult experiments to become feasible. Moreover, with easy access to core facilities or commercial providers of pronuclear injections, generating simple Thy1 transgenic mice is now a possibility even for small laboratories.
WebbCommon Name: GP4.3. These transgenic mice, made by the Genetically-Encoded Neuronal Indicator and Effector ( GENIE) Project, express the green fluorescent calcium indicator, … WebbGCaMP6 expression across brain regions and neurons and compared to other transgenic mice and AAV-mediated expression. We tested three mouse lines for imaging in the …
Webb24 sep. 2014 · We tested three mouse lines for imaging in the visual cortex in vivo and compared their performance to mice injected with AAV expressing GCaMP6. Furthermore, we show that GCaMP6 Thy1...
WebbThe genetically encoded green fluorescent protein-based calcium sensor, GCaMP, has been used to detect calcium transients and report neuronal activity. We evaluated the … incoherent wavesWebb18 okt. 2012 · To generate GCaMP transgenic mice, we used the well-characterized Thy1 promoter to express GCaMPs in neurons. We generated eight founder lines of … incoherently synonymsWebbA new phenotype identification method with the fluorescent expression in cross-sectioned tails in Thy1-GCaMP6s transgenic mice Authors Shutaro Kobayashi 1 2 , Kazunori O'Hashi 1 3 4 , Keisuke Kaneko 1 5 , Satomi Kobayashi 1 3 6 , Shouhei Ogisawa 1 2 , Morio Tonogi 2 , Satoshi Fujita 3 6 , Masayuki Kobayashi 1 3 7 Affiliations incoherently meansWebbGCaMP6 functional connectivity maps of all 7 individual mice in the imaging cohort, filtered over the delta (0.4–4.0Hz) frequency band. The same canonical network seeds used in Figs Figs44and and55were applied here. Data shown are acquired in the awake state. (TIF) pone.0185759.s002.tif(2.8M) GUID: C405C9D5-751C-4285-B16C-4C0455F11153 incokbWebb23 sep. 2024 · Adult (P65-P70) transgenic mice (C57BL/6J-Tg (Thy1-GCaMP6f) GP5.1Dkim/J; The Jackson Laboratory) expressing GCaMP6f under the Thy1 promoter were used. All animals were housed in a temperature-controlled (24 °C) colony room and maintained in a 12-h/12-h light/dark cycle (lights on at 7:00 a.m.). Food and water were … incoinfoWebb20 jan. 2024 · Transgenic Thy1.2-GCaMP6s mice expressing GCaMP6 slow in some DRG neurons have been previously described (61). As no significant differences were observed between male and female mice,... incoindWebb23 juli 2024 · For example, Thy1-GCaMP6 mice show stable expression of GCaMP6 in multiple cortical regions across months without apparent toxicity, but only after the third postnatal week (Dana et al., 2014). Similarly, the Ai38 mouse line expresses GCaMP3 when treated with tamoxifen at P7, but showed very low expression at 4 weeks of age … incois located in